Microscopy in Medicine Lab
The Microscopy in Medicine Core (MiM Core) at Emory University is a core facility providing access to histology and state-of-the-art imaging instrumentation on a shared-use basis to faculty, staff and students of the Emory Division of Cardiology and Emory Department of Medicine. The facility is located in the Woodruff Memorial Research Building (WMB) on the Emory campus. Map to Mim Core facilities.
Contact - Mim Core director, Dr. Lula Hilenski.
Emory MiM Core Image Collage
Please review the MimCore guidelines and policies before starting work in the lab.
Acknowledgment of the Microscopy in Medicine (MiM) Core
Software for Image Analysis
- Bolte Guided Tour
- Costes Coloc Biophys
- Guidelines for Accurate Colocalization
- Guidelines for Publications using Imaris QuickStart Tutorials
3-D Volume Rendering
Image-Pro Plus for measurements of distance, area, intensity
- Tutorial 1 - Counting, Sorting and Categorizing Objects and Images
- Tutorial 2 - Basic Counting of objects with IPP v 6.2 using color
- Tutorial 3 - Background Correction with IPP v 6.2
- Tutorial 4 - Counting nuclei with IPP v 6.2
- Tutorial 5 - Distance Measurements with IPP v 6.2
- Tutorial 6 - How to Put Scale Bars
- Tutorial 7 - Measuring distances and areas with IPP v 6.2
- Tutorial 8 - Measuring areas with IPP v 6.2
AutoDeblur Deconvolution Software
Please review the following methods and protocols for using the microscopy in medicine lab.
- Protocol for immunocytochemistry
- Variables in Immunolabeling
- Nuclei staining
- 39 Steps Critical Aspects of Fluorescence Confocal Microscopy
- IHC Handbook
- Collagen Stains Picrosirius Red
Please review the attached lectures on microscopy and immunostaining.
Please review the attached documents for more information on the Emory University Department of Medicine Hisology Equipment
- Histology Equipment in WMB 301
- Leica TP 1020 Automatic Tissue Processor with vacuum
- Leica EG1160 Tissue Embedding Station Manual
- Leica EG1160 Tissue Embedding Station
- Shandon FSE cryotome for frozen tissue sectioning
- Shandon Cryotome FSE Manual
- Leica CM1950 cryostat for frozen tissue sectioning
- Leica CM1950 Cryostat Manual
- Leica RM2035 and RM 2235 microtomes for paraffin sectioning
- Leica RM2235 Microtome Manual
Please review the documents attached below for more information about Imaging Ethics.
Lula L. Hilenski, PhD
Assistant Professor of Medicine
Department of Medicine/Division of Cardiology
Emory University School of Medicine
Helpful Websites at other Microscopy Imaging Centers
- Albert Einstein College of Medicine -excellent manuals and protocols
- Florida State University - Outstanding interactive tutorials and graphics explaining optics computer-based imaging
- University of Wisconsin-Madison
- Stanford U - Excellent Q&A sections
- Cal Tech
- University of Georgia-Athens
- University of Arizona
- WWW Virtual Library: Microscopy
- Purdue University
Other helpful sites
All potential users of the systems are asked to contact the director of the facility, Dr. Lula Hilenski, to schedule a one-on-one Basic Training session at a mutually convenient time. These individual training sessions, which usually last about 2 hours, include proper use and care of microscope objectives, operation of the microscope and laser system, laser safety, as well as how to acquire, save, export and transfer images.