Lowance Center for Human Immunology
The Lowance Center for Human Immunology, led by Emory Division of Rheumatology Director Ignacio Sanz, MD, is an initiative of the Department of Medicine to cut across traditional academic divisions in medicine and to translate new knowledge in basic immunology to human immune-mediated diseases.
Goals of the Lowance Center
The overall goal of the Lowance Center is to understand the immunological and molecular mechanisms responsible for human autoimmune and allergic diseases. This goal is pursued through the integrated effort of basic, clinical and translational scientists applying state-of-the-art technology as well as advanced epidemiology and outcomes research tools.
In addition to providing fundamental insight into disease pathogenesis, the Lowance Center aims to identify specific immunological defects that can be used to develop biomarkers of disease heterogeneity in order to better segment diseases into discreet subsets. In turn, disease segmentation will be used to select treatments targeted to the defective molecular pathways specifically responsible for different disease subsets. This approach should result in more effective and safer treatments for the individual patient. The knowledge derived from our studies will also provide better biomarkers of disease progression and response to treatment. Ultimately, we seek to use this knowledge to reverse well-established disease and to prevent disease development in high-risk subjects.
Emory University has an outstanding faculty performing research in basic immunology and providing care for patients with immune-mediated inflammatory diseases.
Research and Clinical Faculty
The Lowance Center contributes to improving immunological knowledge and advancing the treatment of human immunological diseases through the coordinated effort of dedicated scientists and physicians working together with clinical coordinators and nurses.
The main research interest in the Ignacio Sanz Lowance Center laboratory is to investigate how the immune system goes awry in autoimmune diseases, with a particular emphasis on the roles of B cells in the pathogenesis of systemic lupus erythematosus (SLE).
Efforts in the lab focus on getting an in-depth knowledge of the diversity, function and regulation of human B cells, which is fundamental to the understanding of autoimmune diseases. The ultimate goal is to be able to apply this knowledge to the identification of new targets and the development of more effective strategies in B-cell directed therapy for autoimmune diseases.
Recognizing the importance of properly identifying the diverse B cell subsets in studying their function and regulation, we have undertaken a comprehensive B cell phenotyping approach using polychromatic flow cytometry. With this approach, we are able to not only recognize the disturbances of B cell homeostasis in a number of human diseases, but also identify several novel B cell and plasma cell subsets that are disregulated in disease
Over the last few years, we have incorporated several cutting-edge technologies to advance our research inquiries. For example, Next Gen Sequencing (NGS) enables us to scrutinize the diversity and clonal make-up of the antibody repertoire expressed by sorted B cell populations. Of particular interest is to understand the origin and composition of circulating plasmablasts in SLE during acute flares, since SLE is a prototypic autoantibody-mediated disease in which disease exacerbations are characterized by profound ASC abnormality. Interrogations of the repertoire and clonal relationship between plasmablasts and other B cell subsets in the circulation as well as resident bone marrow plasma cells in a temporal fashion will allow us to elucidate the origin, diversification, and maturation of the B cell response in a chronic, systemic autoimmune setting. In addition to exogenous factors that have been shown to cause B cell disregulation in SLE, it is postulated that altered epigenetic programming of autoreactive B cells causes their activation and recruitment to the effector compartment, resulting in autoantibody production and disease pathogenesis. This hypothesis is currently being addressed in collaboration with Jerry Boss’ lab first by searching for key genes involved in the aberrant activation of B cell subsets in SLE through global epigenetic profiling and transcriptional profiling analyses using MeDIP-Seq and RNA-Seq, respectively.
Studies of the initial breakdown of tolerance and the subsequent expansion of pathogenic B cells are hampered by challenges in the identification of disease-specific autoreactive B cells in SLE. To circumvent these limitations, my lab has employed a subset of B cells that is recognized by an anti-idiotype 9G4 antibody. Given their autoreactivity against multiple lupus antigens and the shared expression of VH4-34 gene, the study of 9G4+ cells represents a highly informative experimental model to understand the breakdown of B cell tolerance in SLE. In healthy subjects, effective tolerance ensures that 9G4 responses are restricted to acute infections with Mycoplasma and EBV, and that they do not persist in the long-lived IgG memory and plasma cell compartments. In contrast, we have shown that 9G4+ B cells are substantially expanded in these compartments in SLE owing to defective germinal center censoring that is unique to SLE among the autoimmune diseases. The 9G4 model is also instrumental in identifying the autoreactivity at a clonal level. Conventionally, this has to rely on the reconstruction of individual recombinant monoclonal antibodies from sorted single cells, which is a time-consuming and expensive process. We have collaborated with Dr. Georgiu’s group (UT Austin) to incorporate his large throughput technologies for the interrogation of >100,000 single cells/sample using microfluidics and emulsion-based PCR for the amplification of linked VH and VL genes and the generation of selected monoclonal antibodies. These approaches will enable the identification of autoantigens and investigation of their relative roles in the initiation of tolerance breakdown and selection of pathogenic B cells that culminate in disease manifestation.
Professor of Medicine and Pediatrics
Mason I. Lowance Chair of Allergy and Immunology
Director, Lowance Center for Human Immunology
Director, Division of Rheumatology
Georgia Research Alliance Eminent Scholar in Immunology
Interim Senior Business Manager
- Emily Blalock, PhD, Post-Doctoral Fellow
- Kevin Cashman, PhD, Post-Doctoral Fellow
- Asiya Chida, PhD, Post-Doctoral Fellow
- So-Hee Hong, PhD, Post-Doctoral Fellow
- Louise Hartson, Research Specialist, Senior
- Hinel Patel, Clinical Research Coordinator
- Kira Smith, PhD, Lead Research Specialist
- Anna Stephens, Clinical Research Coordinator
- Deepak Tomar, PhD, Associate, Research Track
- Xiaoqian Wang, PhD, Associate, Research Track
- Youliang Wang, Research Specialist, Sr.
247 Whitehead Biomedical Research Building
615 Michael Street
Atlanta, Georgia 30322
For a more complete list of publications please visit PubMed
- Sanz. I. Pharmacological Effects and Mechanisms of Action of Agents Blocking B cells. In: Bosch X, Ramos-Casals M, Khamashta MA (eds). Drugs Targeting B-cells in Autoimmune Diseases. Springer: Basel, 2014, pp 37-65.
- Sanz I. Rationale for B cell targeting in SLE. Semin Immunopathol 2014, 36(3):365-375.
- Sanz I, Yasothan U, Kirkpatrick P. Belimumab. Nat Rev Drug Discov 2011, 10(5):335-336.
- Sanz I. Connective tissue diseases: Targeting B cells in SLE: good news at last! Nat Rev Rheumatol 2011, 7(5): 255-256.
- Sanz I, Lee FE. B cells as therapeutic targets in SLE. Nat Rev Rheumatol 2010,6(6): 326-337.
- John Looney R, Anolik J, Sanz I. A perspective on B-cell-targeting therapy for SLE.Modern Rheumatology 2010, 20(1): 1-10.
- Manjarrez-Orduno N, Quach TD, Sanz I. B Cells and Immunological Tolerance. J Invest Dermatol 2009, 129(2): 278-288.
- Sanz I, Anolik JH, Looney RJ. B cell depletion therapy in autoimmune diseases.Front Biosci 2007, 12: 2546-2567
- Looney RJ, Anolik JH, Campbell D, Felgar RE, Young F, Arend LJ, Sloand J, Rosenblatt J, Sanz I. B cell depletion as a novel treatment for systemic lupus erythematosus: A phase I/II dose-escalation trial of rituximab. Arthritis & Rheumatism2004, 50(8): 2580-2589.
- Looney RJ, Anolik J, Sanz I. B cells as therapeutic targets for rheumatic diseases.Curr Opin Rheumatol 2004, 16(3): 180-185.
- Anolik JH, Barnard J, Cappione A, Pugh-Bernard A, Felgar RE, Looney RJ, Sanz I. Rituximab improves peripheral B cell abnormalities in human systemic lupus erythematosus. Arthritis & Rheumatism 2004, 50(11): 3580-3590.
- Anolik J, Sanz I. B cells in human and murine systemic lupus erythematosus. Curr Opin Rheumatol 2004, 16(5): 505-512.
- Anolik JH, Campbell D, Felgar RE, Young F, Sanz I, Rosenblatt J, Looney RJ. The relationship of FcgammaRIIIa genotype to degree of B cell depletion by rituximab in the treatment of systemic lupus erythematosus. Arthritis Rheum 2003, 48(2): 455-459.
- Roberts MEP, Kaminski D, Jenks SA, Maguire C, Ching K, Burbelo PD, Iadarola M, Rosenberg A, Coca A, Anolik J, Sanz I. Primary Sjögren's Syndrome is characterized by distinct phenotypic and transcriptional profiles of IgD+ unswitched memory cells. Arthritis & Rheumatology 2014: n/a-n/a.
- Kaminski DA, Wei C, Qian Y, Rosenberg AF, Sanz I. Advances in human B cell phenotypic profiling. Frontiers in immunology 2012, 3: 302.
- Kaminski D, Wei C, Rosenberg A, Lee FE-H, Sanz I. Multiparameter Flow Cytometry and Bioanalytics for B Cell Profiling in Systemic Lupus Erythematosus. In: Perl A (ed). Autoimmunity, vol. 900. Humana Press, 2012, pp 109-134.
- Barr PM, Wei C, Roger J, Schaefer-Cutillo J, Kelly JL, Rosenberg AF, Jung J, Sanz I, Friedberg JW. Syk inhibition with fostamatinib leads to transitional B lymphocyte depletion.Clinical Immunology 2012, 142(3): 237-242.
- Wei C, Jung J, Sanz I. OMIP-003: Phenotypic analysis of human memory B cells.Cytometry Part A 2011: n/a-n/a.
- Quách TD, Manjarrez-Orduño N, Adlowitz DG, Silver L, Yang H, Wei C, Milner ECB, Sanz I. Anergic Responses Characterize a Large Fraction of Human Autoreactive Naive B Cells Expressing Low Levels of Surface IgM. The Journal of Immunology 2011, 186(8):4640-4648.
- Qian Y, Wei C, Eun-Hyung Lee F, Campbell J, Halliley J, Lee JA, Cai J, Kong YM, Sadat E, Thomson E, Dunn P, Seegmiller AC, Karandikar NJ, Tipton CM, Mosmann T, Sanz I, Scheuermann RH. Elucidation of seventeen human peripheral blood B-cell subsets and quantification of the tetanus response using a density-based method for the automated identification of cell populations in multidimensional flow cytometry data. Cytometry Part B: Clinical Cytometry 2010, 78B(S1): S69-S82.
- Newell KA, Asare A, Kirk AD, Gisler TD, Bourcier K, Suthanthiran M, Burlingham WJ, Marks WH, Sanz I, Lechler RI, Hernandez-Fuentes MP, Turka LA, Seyfert-Margolis VL. Identification of a B cell signature associated with renal transplant tolerance in humans.The Journal of Clinical Investigation 2010, 120(6): 1836-1847.
- Palanichamy A, Barnard J, Zheng B, Owen T, Quach T, Wei C, Looney RJ, Sanz I, Anolik JH. Novel human transitional B cell populations revealed by B cell depletion therapy.J Immunol 2009, 182(10): 5982-5993.
- Wei C, Anolik J, Cappione A, Zheng B, Pugh-Bernard A, Brooks J, Lee EH, Milner EC, Sanz I. A new population of cells lacking expression of CD27 represents a notable component of the B cell memory compartment in systemic lupus erythematosus. J Immunol 2007, 178(10): 6624-6633
- Richardson C, Chida AS, Adlowitz D, Silver L, Fox E, Jenks SA, Palmer E, Wang Y, Heimburg-Molinaro J, Li QZ, Mohan C, Cummings R, Tipton C, Sanz I. Molecular basis of 9G4 B cell autoreactivity in human systemic lupus erythematosus. J Immunol 2013,191(10): 4926-4939.
- Pugh-Bernard AE, Silverman GJ, Cappione AJ, Villano ME, Ryan DH, Insel RA, Sanz I. Regulation of inherently autoreactive VH4-34 B cells in the maintenance of human B cell tolerance. J Clin Invest 2001, 108(7): 1061-1070.
- Cappione AJ, Pugh-Bernard, A. E., Sanz, I. Lupus VH4.34-Encoded Antibodies Bind to a B220-Specific Glycoform of CD45 on the Surface of Human B Lymphocytes. Arthritis & Rheumatism 2002, 46(9): S222-S223.
- Cappione AJ, Pugh-Bernard AE, Anolik JH, Sanz I. Lupus IgG VH4.34 antibodies bind to a 220-kDa glycoform of CD45/B220 on the surface of human B lymphocytes. J Immunol 2004, 172(7): 4298-4307.
- Cappione A, 3rd, Anolik JH, Pugh-Bernard A, Barnard J, Dutcher P, Silverman G, Sanz I. Germinal center exclusion of autoreactive B cells is defective in human systemic lupus erythematosus. J Clin Invest 2005, 115(11): 3205-3216.
- Milner EC, Anolik J, Cappione A, Sanz I. Human innate B cells: a link between host defense and autoimmunity? Springer Semin Immunopathol 2005, 26(4): 433-452.
- Kobie JJ, Alcena DC, Zheng B, Bryk P, Mattiacio JL, Brewer M, LaBranche C, Young FM, Dewhurst S, Montefiori DC, Rosenberg AF, Feng C, Jin X, Keefer MC, Sanz I. 9G4 Autoreactivity Is Increased in HIV-Infected Patients and Correlates with HIV Broadly Neutralizing Serum Activity. PLoS ONE 2012, 7(4): e35356.
- Alcena DC, Kobie JJ, Kaminski DA, Rosenberg AF, Mattiacio JL, Brewer M, Dewhurst S, Dykes C, Jin X, Keefer MC, Sanz I. 9G4+ antibodies isolated from HIV-infected patients neutralize HIV-1 and have distinct autoreactivity profiles. PLoS One 2013, 8(12):e85098.
- Jenks SA, Palmer EM, Marin EY, Hartson L, Chida AS, Richardson C, Sanz I. 9G4+ Autoantibodies Are an Important Source of Apoptotic Cell Reactivity Associated With High Levels of Disease Activity in Systemic Lupus Erythematosus. Arthritis & Rheumatism 2013,65(12): 3165-3175.
Autoimmune Centers of Excellence
The National Institute of Allergy and Infectious Diseases (NIAID) has created the Autoimmunity Centers of Excellence (ACEs) to encourage and enable collaborative research – across scientific disciplines, across medical specialties, and between basic and clinical scientists – in the search for effective treatments for autoimmune diseases.
Georgia Resource Alliance
The Georgia Research Alliance (GRA) grows Georgia's economy by expanding university research capacity and by seeding and shaping startup companies around inventions and discoveries.